Trivia about Superman/Batman: Public Enemies.
- In the fight at the beginning between Metallo, Superman and Batman in the cemetery one of the headstones reads "here lies stu deo adaelaide 1996 - 2008"
- Tim Daly, Kevin Conroy, and Clancy Brown are no strangers to their roles in this movie. Conroy has done the voice of Batman in every major animated Batman series (except for The Batman and Batman: the Brave and the Bold and The Batman where he did cameo as John Grayson, father of Dick Grayson) as well as voicing the character in both Justice League and Static Shock. Daly and Brown did the voices of Superman and Lex Luther in Superman: The Animated Series, and Brown also portrayed Luther in Justice League.
- Allison Mack, who voices Powergirl, is no stranger to working in a Superman production as she has played the part of Chloe Sullivan in the WB's "Smallville" (2001) throughout the entire series.
- This is one of the few Superman-oriented animated projects connected to producer Bruce W. Timm and Andrea Romano where Lois Lane made a cameo appearance, but with no lines. This is another break away from the original story, where Lois aided Superman and Batman in distracting Lex Luthor with a televised interview while they made their way towards the White House to confront him. However, it is indicated in the film twice that Lois, at this stage, is aware of his secret identity and are in a relationship.
- Before Superman and Batman face off against the hoard of villains, Batman tells Superman "Your funeral" which Superman responds with "Already had one." Superman is referring to the "Death of Superman" storyline which was adapted into the first DC Universe movie Superman/Doomsday.
- In the behind-the-scenes feature, which appears on the Green Lantern: First Flight DVD, voice casting director Andrea Romano said that she fought hard to get her original cast together again for this movie. She was referring to Kevin Conroy as Batman, Tim Daly as Superman, and Clancy Brown as Lex Luthor.
- Much of the voice-over work was done with the actors in the same room in order to let them play off of one another.
- Although they are mentioned by name, Superman and Batman's alter egos, Clark Kent and Bruce Wayne respectively, do not make an appearance.
- In the original comic storyline by Jeph Loeb and Ed McGuinness, it was Captain Atom, not Batman, who piloted the Superman-Batman Composite rocket to destroy the kryptonite asteroid. And unlike Batman, Captain Atom did not survive the devastation. The meteor story was continued in the second arc, as the broken fragments not only fell to earth in multiple versions of kryptonite, but also the ship carrying Superman's cousin, Kara Zor-El (Supergirl). The storyline, also written by Loeb, served to be one of the last projects penciled by the late artist, Michael Turner.